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41.
The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological
specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine
bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed
by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent
microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration
and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were
flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based
on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is
easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement
of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as
a new tool to study the fine structures of marine bacteria.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
42.
Nicolas Mazzella Josiane Molinet Agung Dhamar Syakti Jean-Claude Bertrand Pierre Doumenq 《Marine Chemistry》2007,103(3-4):304-317
This in situ study reports on the relevance of intact phospholipids as new biomarkers and highlights the limits of the classical fatty acid analysis after the hydrolysis of the polar lipid fraction. The analysis of polar lipid fractions revealed significant contributions of marine macrophytes with either non-phospholipid compounds (sulfoquinovosyl diacylglycerol) or with both non-bacterial phospholipid classes (phosphatidylcholine) and non-bacterial phospholipid molecular species (some molecular species of phosphatidylglycerol). The analysis of intact phospholipids showed also the predominance of gram-negative bacteria in sediments whereas the analysis of fatty acids alone cannot confirm such information. Lastly, as regard to the effects of petroleum hydrocarbons on the sedimentary bacterial communities, we observed a higher degree of saturation of the fatty acyl chains of the phospholipids extracted from sediment exposed to a massive and chronic crude oil contamination. Such a result was interpreted as an adaptive response resulting in higher membrane rigidity of the bacterial communities. 相似文献
43.
秋季东、黄海异养细菌(Heterotrophic Bacteria)的分布特点 总被引:27,自引:1,他引:27
2000年10-11月,乘"北斗号"考察船进行秋季"东、黄海生态系统动力学与生物资源可持续利用"大面调查,研究秋季东、黄海异养细菌的分布.结果表明,异养细菌在黄海和东海的丰度分别在(2.37-13.33)×108 cell/L和(3.05-13.62)×108 cell/L之间.细菌丰度最高值出现在长江口附近,且断面E和断面F各站位的细菌丰度明显高于其他断面.异养细菌丰度大小以长江口为中心向外海依次递减.东、黄海水体异养细菌生物量分别在244.45-1812.90mgC/m2和100.60-940.87mgC/m2之间.东、黄海异养细菌丰度无显著差异,但是东海海域水体异养细菌生物量高于黄海海域.异养细菌空间分布与浮游植物叶绿素在东海有一定的相关性,黄海异养细菌与硝酸盐浓度的相关性极显著. 相似文献
44.
Biological Responses to Seasonally Varying Fluxes of Organic Matter to the Ocean Floor: A Review 总被引:8,自引:0,他引:8
Andrew J. Gooday 《Journal of Oceanography》2002,58(2):305-332
Deep-sea benthic ecosystems are sustained largely by organic matter settling from the euphotic zone. These fluxes usually
have a more or less well-defined seasonal component, often with two peaks, one in spring/early summer, the other later in
the year. Long time-series datasets suggest that inter-annual variability in the intensity, timing and composition of flux
maxima is normal. The settling material may form a deposit of “phytodetritus” on the deep-seafloor. These deposits, which
are most common in temperate and high latitude regions, particularly the North Atlantic, evoke a response by the benthic biota.
Much of our knowledge of these responses comes from a few time-series programmes, which suggest that the nature of the response
varies in different oceanographic settings. In particular, there are contrasts between seasonal processes in oligotrophic,
central oceanic areas and those along eutrophic continental margins. In the former, it is mainly “small organisms” (bacteria
and protozoans) that respond to pulsed inputs. Initial responses are biochemical (e.g. secretion of bacterial exoenzymes)
and any biomass increases are time lagged. Increased metabolic activity of small organisms probably leads to seasonal fluctuations
in sediment community oxygen consumption, reported mainly in the North Pacific. Metazoan meiofauna are generally less responsive
than protozoans (foraminifera), although seasonal increases in abundance and body size have been reported. Measurable population
responses by macrofauna and megafauna are less common and confined largely to continental margins. In addition, seasonally
synchronised reproduction and larval settlement occur in some larger animals, again mainly in continental margin settings.
Although seasonal benthic responses to pulsed food inputs are apparently widespread on the ocean floor, they are not ubiquitous.
Most deep-sea species are not seasonal breeders and there are probably large areas, particularly at abyssal depths, where
biological process rates are fairly uniform over time. As with other aspects of deep-sea ecology, temporal processes cannot
be encapsulated by a single paradigm. Further long time-series studies are needed to understand better the nature and extent
of seasonality in deep-sea benthic ecosystems.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
45.
利用平板涂布法从南海海绵生物样品中分离海绵共栖细菌,以枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌及酿酒酵母作为实验用指示菌对海绵共栖细菌进行了抗菌活性筛选。利用气相质谱联用仪(GC-MS)建立了群体感应信号分子酰基高丝氨酸内酯类化合物(AHLs)的检测方法,分析了活性菌株粗提液中信号分子的产生及种类。同时采用16S rDNA同源性和系统发育分析对活性菌株进行种属鉴定和系统发生学研究。结果表明,在分离到的94株海绵共栖细菌中18株(占19.1%)细菌具有抗菌活性。通过GC-MS检测确定大多数活性菌株都含有AHLs,因此提出了海绵共栖细菌抗菌活性与群体感应之间可能存在必然联系的推测。通过细菌分类鉴定,结果显示具有抗菌活性的细菌大部分属于芽孢杆菌属(Bacillus)。 相似文献
46.
47.
牙鲆(Paralichthys olivaceus)秦皇岛弧菌感染症及其病原细菌研究 总被引:1,自引:1,他引:1
采用病原分离与鉴定、人工感染试验等方法,在对养殖牙鲆病例进行发病情况、病理变化特征检验的基础上,进行病原学检验。结果表明,此病例属于由弧菌属(VibrioPacini1854)细菌引起的败血感染症。经对12株纯培养菌(HQ010712-1—HQ010712-12)进行形态特征、理化特性等表观分类学指征的检验,选择代表菌株(HQ010712-1株)进行16S rRNA基因的测定与系统发育学分析等,判定为弧菌属的一个新种。将HQ010712-1株送中国典型培养物保藏中心(CCTCC)进行了复核鉴定与分类定名,依据分离地定名为秦皇岛弧菌(Vibrioqinhuangdaorasp.nov.);参考菌株为HQ010712-1。 相似文献
48.
白令海夏季浮游细菌和原生动物生物量及分布特征 总被引:4,自引:0,他引:4
1999年7月21日至8月1日在我国首次北极科学考察期间,考察了白令海中部的浮游细菌和原生动物,分析了其丰度、分布、生物量及其生态作用,结果显示,浮游细菌表层生物量为1.5~20.2μg/dm3,平均为浮游植物生物量的30%,100m以上水柱柱总生物量(720~3123mg/m2)平均为浮游植物柱总生物量的67%,因而是白令海夏季与浮游植物处同等量级的优势类群;原生动物表层生物量为1.2~27.4μg/dm3,100m以上水柱柱总生物量为189~1698mg/m2,平均为浮游植物柱总生物量的21%,其中粒径小于5,5~20μm和大于20μm的原生动物分别占其柱总生物量的13%,47%和40%;作为主要类群的异养腰鞭毛虫占原生动物柱总生物量的39%.浮游细菌和原生动物生物量的总体分布趋势从西部向东北和东部递减、从表层向深层衰减,20~25m水层温跃层和表层海流的存在对这一分布特性可能有较大的影响.原生动物受潜在的大、中型浮游动物捕食压力的制约,维持了一个相对较低的生物量水平,在一定程度上限制了微食物环(microbial food loop)在该海域夏季生态系统营养中的作用. 相似文献
49.
采用麻痹性贝类毒素小白鼠生物测定法 ,研究了在可控生态条件下两株海洋细菌S1 0 、P42 对塔玛亚历山大藻生长及其产毒量的影响。结果表明 ,菌株S1 0 在较高浓度下对藻细胞的生长有明显的抑制作用 ,在较低浓度下抑藻生长作用较弱 ,不同浓度的菌株S1 0 均能有效地抑制藻细胞内麻痹性贝类毒素的产生 ,且在较低浓度下效果较好 ;菌株P42 对该藻的作用恰好与S1 0 相反 ,在较低浓度下明显抑制藻细胞的生长 ,不同浓度的菌株P42 也能有效地抑藻产毒 ,且在较高浓度下作用较明显。实验用的藻株毒力约为 ( 0 .95— 1 2 .1 4)× 1 0 - 6MU/cell,属于低毒藻株 ,该藻株在培养第 1 4天达到毒性最高峰 ,峰值为 1 2 .1 4× 1 0 - 6MU/cell,之后逐渐下降。讨论了海洋细菌在赤潮生物防治中的应用前景 相似文献
50.